Peptone stock solutions were prepared 20%, wv, sterilized by filtration through 0. Animalfree peptones and yeast extracts bd biosciences. This item has been discontinued and is no longer available. Bacto peptone is used as an organic nitrogen source in microbiological culture media for cultivation of a variety of bacteria and fungi. Below is a list of some of the peptones that are used as ingredients in. Bacto peptone was first introduced in 1914 and became the standard peptone for the preparation of bacteriological culture media. Lysis of halobacteria in bactopeptone by bile acids ncbi nih. Protein and carbohydrate composition of the cell envelope of halobacterium. It is a modification of proteose peptone adapted for use in the preparation of chocolate agar for propagation of neisseria species and chocolate tellurite agar for corynebacterium diphtheriae. Customers have successfully used this supplement with a variety of cell types including saccharomyces cerevisiae, escherichia coli, pasteurella multocida, vibrio cholerae, and porcine kidney cells to produce biotherapeutics and vaccines for animal health.
The nutritive value of bacto peptone is largely dependent on a combination of a negligible quantity of proteases, high concentration of complex constituents, and a unique amino acid content that makes this peptone a strong nitrogen source. The limitations of lb medium small things considered. Becton dickinson microbiology 211677 bacto peptone, 500 g volume. Bacto proteose peptone and bacto yeast extract, originally manufactured by difco, are now available directly from bd sciences bd diagnostic systems no211684 and bd diagnostic systems no212750, respectively and through retailers like fisher scientific. The nutritive value of bacto peptone is largely dependent on the amino acid content that supplies essential nitrogen. Peptone, special 23 gl agar 12 gl sodium chloride 5 gl. Autoclave components for a maximun of 15 minutes at 121c. Essential agents for you to prepare your culture media. Bacto peptone is an essential agent to prepare your culture media. Fermentation free fulltext use of chicken feather peptone and. In rich axenic media, pp is often supplemented by varying concentrations of yeast extract, glucose, and some form of iron. The dark blue bar reflects the chemically defined cd control medium. Proteose peptone pp, an enzymatic digest of animal protein high in proteoses, is the traditional basis for most media used for growing tetrahymena in the laboratory.
In their definitive 1968 study of bacto neopeptone using gel filtration. Bd difco bacto peptone, is derived from an enzymatic digest of animal protein. Bacto proteose peptone and bacto yeast extract, originally manufactured by difco. Proteose peptones an overview sciencedirect topics. In a protocol, it was said to use bactotryptone and we have tryptone. Animal free peptones and yeast extracts aspartic acid % total cystine % free glutamic acid % free. Due to the complex composition of peptones, they provide a wide range of benefits to. The light blue bar shows the peptone supplementing the control media and the gray and yellow show the cd media supplement. Manual of microbiological culture media becton dickinson. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Substances in bactopeptone which caused lysis of halobacteria were purified.
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